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Image Search Results
Journal: International Journal of Nanomedicine
Article Title: Graphene Oxide Nanosheets Induce Mitochondrial Toxicity in Human Ovarian Granulosa Cells: Implications for Female Reproductive Health
doi: 10.2147/IJN.S495259
Figure Lengend Snippet: Cytotoxic effects of graphene oxide nanosheets on KGN cells. ( A ) Human ovarian granulosa cells were cultured and exposed to graphene oxide nanosheets (0.1–100 μg/mL) at varying concentrations for 48 h and 72 h, respectively. Subsequently, a CCK-8 assay was conducted to determine the percentage of viable cells that were expressed. ( B ) Flow cytometry was used to analyze apoptosis in KGN cells treated with Annexin V-FITC/PI after 72 h of different treatments. ( C ) Quantitative analysis of the ratio of apoptosis and cell necrosis in ( B ). Data are expressed as Mean ± SEM (n=3). Statistical differences between experimental groups were analyzed by the Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
Article Snippet: The
Techniques: Cell Culture, CCK-8 Assay, Flow Cytometry